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KMID : 0615720090260060067
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Jouranl of Korean Acupuncture and Moxibustion society
2009 Volume.26 No. 6 p.67 ~ p.72
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Effects of Radix Aconiti Herbal Acupuncture Injected at Hwando(GB30) on Neuropathic Pain in Rats
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Bang Sung-Pil
Ryu Mi-Seon
Kim Jae-Hong
Wei Tung-Sheun
Youn Dae-Hwan
Yoon Yeo-Choong
Yeo Kyeong-Chan
Jung Ho-Seok
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Abstract
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Objectives : The purpose of this study is to examine if Radix Aconiti herbal acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain.
Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, L5 spinal nerve was ligated by 6-0 silk thread. After neurophathic surgery, the author examined if the animals exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the medial malleolus with von frey filament and acetone. Three days after the neuropathic surgery, Radix Aconiti herbal acupuncture was injected at Hwando(GB30) one time per two days for 2 weeks. After that, the author examined the withdrawal response of neuropathic rats’ legs by von frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats.
Results : The Radix Aconiti herbal acupuncture injected Hwando(GB30) decreased the withdrawal response of mechanical allodynia that assessed with von frey filament in RA1-GB30, RA2-GB30 group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando(GB30) decreased the withdrawal response of chemical allodynia(cold allodynia) that assessed with acetone in RA1-GB30, RA2-GB30 group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando(GB30) showed the significant between control group and RA2-GB30 group in the c-Fos expression. The Radix Aconiti herbal acupuncture injected Hwando(GB30) did not show the significant between control group and RA1-GB30 group, RA2-GB30 group and RA3-GB30 group in the WBC, RBC, HGB, HCT count.
Conclusions : We have noticed that Radix Aconiti herbal acupuncture at Hwando(GB30) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-fos expression in the central gray of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of pain.
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KEYWORD
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Neuropathic pain, Radix Aconiti Herbal Acupuncture, Hwando(GB30), Allodynia, c-Fos
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